Coding

Part:BBa_K4585005:Design

Designed by: Zijun Zhang   Group: iGEM23_CSU-CHINA   (2023-09-24)


Degron


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 92
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 92
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 92
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The degron we used is C-terminal of short-lived proteins not N-terminal.


Source

Degron were discovered at the C-terminal of short-lived proteins.

References